Pharmaceutical Sciences

Dr. Payel Datta earned her Ph.D. in Life Sciences from Rensselaer Polytechnic Institute (RPI, NY), and her Ph.D. work focused on "Metabolic engineering mammalian cells for the production of value-added-biomolecules". Her postdoctoral work at Albany Medical College focused on developing and utilizing a 3D cell-based assay. Dr. Datta's work at Heparin Applied Research Center (start-up Industry-RPI, NY) focused on process development, scale-up, and tech transfer of value-added products (e.g., enzymes, small molecules, anticoagulant drug heparin, probiotic bacteria). In addition, her research focused on drug efficacy and toxicity studies. Dr. Datta has coauthored a book chapter in the “Encyclopedia of Microbiology”, numerous publications in peer-reviewed journals, presented work at conferences, and authored technical documents. Dr. Datta is highly motivated to harness her expertise and knowledge in teaching, research, and workforce development.

Education

• Ph.D., Biology, Rensselaer Polytechnic Institute, Troy, NY, 2013

Academic Courses Taught at ACPHS (2021-Present)

• BIO 331/631G            Mammalian Cell Culture
• BIO 348/648G            Microbial Fermentation
• BIO 675G                   Biopharmaceutical Capstone
• PSC 320/620G         Downstream Processing of Biopharmaceutical Products
• PSC 646G                 Regulatory Science

Workforce Development and Training Programs at CBET (2021-Present)

• Principles of Biomanufacturing
• Mammalian Cell Culture: From cell bank to bioreactor
• NIIMBL eXperience at ACPHS
• FDA mRNA Training Program at ACPHS
• Modifications

Current Research Interests
Drug safety and efficacy, Cell-based screening platforms, Biopharmaceuticals, and Small-molecule drugs.

Medicines aim to prevent or treat diseases. Medicines comprise diverse drug products, including biopharmaceuticals and small-molecule drugs. Biopharmaceuticals are biomolecules produced using cell systems. Small-molecule drugs are synthesized chemically. The journey a medicine/drug takes from its discovery to its approval is highly regulated. The most critical questions include whether the drug is safe and effective. Our research aims to explore the molecular mechanisms of drug action. Specifically, we employ cell-based screening platforms (2D and 3D cell culture systems) to examine the effects of chemicals and biomolecules on cellular processes, including cell growth, metabolism, migration, signaling, and gene expression. Additionally, we aim to investigate the impact of intrinsic and extrinsic factors on drug quality. Through these combinatorial approaches, the research aims to enhance the understanding of the molecular mechanisms underpinning drug efficacy and potential toxicity.

Additional Research Interests

• Understanding the biology of industrially relevant cell systems and emerging/alternate chassis
• Engineering cells to produce value-added products
• Evaluating how intrinsic and extrinsic factors impact product quality, and productivity
• Value-added Polysaccharides
• Monoclonal antibodies

Journal Review

• Biomolecules
• Biotechnology and Bioengineering
• Process Biochemistry

Book Chapter

• Industrial production of glycosaminoglycans. P. Datta, R. J. Linhardt, S. T. Sharfstein. In Encyclopedia of Microbiology https://www.sciencedirect.com/science/article/pii/B9780128096338122241

Review Articles

• Biosynthetic polysaccharide-based drugs and nutraceuticals from non-animal sources. P. Datta, R. J. Linhardt, Drug Target Review. 2020, 7:41-43 https://www.sciencedirect.com/science/article/pii/B9780128096338122241
• An ‘omics approach towards CHO cell engineering. P. Datta, R. J. Linhardt, S. T. Sharfstein. Biotechnology and Bioengineering. pubmed.ncbi.nlm.nih.gov/23322664/

Selected List of Publications

• Anti-SARS-CoV-2 activity of rhamnan sulfate from Monostroma nitidum. Y. Song, P. He, A. L. Rodrigues, P. Datta, R. Tandon, J. T. Bates, M. Bierdeman, C. Chen, J. S. Dordick, F. Zhang, R. J. Linhardt, Marine Drugs. pubmed.ncbi.nlm.nih.gov/34940684/
• Evaluating heparin products for heparin-induced thrombocytopenia using Surface Plasmon Resonance. F. Zhang, P. Datta, J. S. Dordick, R. J. Linhardt, Journal of Pharmaceutical Sciences. pubmed.ncbi.nlm.nih.gov/31705871/
• Platelet factor 4 polyanion immune complexes: Heparin induced thrombocytopenia and vaccine-induced immune thrombotic thrombocytopenia. P. Datta, F. Zhang, J. S. Dordick, R. J. Linhardt, Thrombosis Journal. pubmed.ncbi.nlm.nih.gov/34526009/
• Complete biosynthesis of a sulfated chondroitin in Escherichia coli. A. Badri, A. Williams, A. Awofiranye, P. Datta, K. Xia, W. He, K. Fraser, J. S. Dordick, R. J. Linhardt, M.A.G. Koffas, Nature Communication. nature.com/articles/s41467-021-21692-5
• Heparosan chain characterization: sequential depolymerization of E. coli K5 heparosan by a bacterial eliminase heparin lyase III and a bacterial hydrolase heparanase Bp to prepare defined oligomers. P. Datta, L. Yan, A. Awofiranye, J. S. Dordick, R. J. Linhardt, Biotechnology Journal. pubmed.ncbi.nlm.nih.gov/33006278/
• High cell density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modifications. P. Datta, L. Fu, P. Brodfuerer, J. S. Dordick, R. J. Linhardt, Applied Microbiology and Biotechnology. https://pubmed.ncbi.nlm.nih.gov/33481068/
• Enzymatic generation of highly anticoagulant bovine intestinal heparin. L. Fu, K. Li, D. Mori, M. Hirakane, L. Lin, N. Grover, P. Datta, Y. Yu, J. Zhao, F. Zhang, M. Yalcin, S. Mousa, J. S. Dordick, R. J. Linhardt, Journal of Medicinal Chemistry. pubmed.ncbi.nlm.nih.gov/28972371/
• Expression of enzymes for 3'-phosphoadenosine-5'-phosphosulfate (PAPS) biosynthesis and their preparation for PAPS synthesis and regeneration. P. Datta, L. Fu, W. He, M. A. G. Koffas, J. S. Dordick, R. J. Linhardt, Applied Microbiology and Biotechnology. https://pubmed.ncbi.nlm.nih.gov/32601738/
• Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin. J. Y. Baik, H. Dahodwala, E. Oduah, L. Talman, T. R. Gemmill, L. Gasimli, P. Datta, B. Yang, G. Li, F. Zhang, L. Li, R. J. Linhardt, A. M. Campbell, S. F. Gorfien, S. T. Sharfstein, Biotechnology Journal. pubmed.ncbi.nlm.nih.gov/26037948/
• Microarray platform affords improved product analysis in mammalian cell growth studies. P. Datta, L. Meli, L. Li, N. Migliore, E. Schaefer, S. T. Sharfstein, J. S. Dordick, R. J. Linhardt, Biotechnology Journal. pubmed.ncbi.nlm.nih.gov/24227746/
• Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells. P. Datta, B. Yang, R. J. Linhardt, S. T. Sharfstein, Cytotechnology. https://pubmed.ncbi.nlm.nih.gov/24468831/
• Bioengineered Chinese hamster ovary cells with Golgi-targeted 3-O-sulfotransferase-1 biosynthesize heparan sulfate with an antithrombin-binding site. P. Datta, G. Li, B. Yang, X. Zhao, J. Y. Baik, T. R. Gemmill, S. T. Sharfstein, R. J. Linhardt, Journal of Biological Chemistry. pubmed.ncbi.nlm.nih.gov/24247246/
• Microscale separation of heparosan, heparan sulfate, and heparin. X. Zhao, B. Yang, K. Linkens, P. Datta, A. Onishi, F. Zhang, R. J. Linhardt, Analytical Biochemistry. pubmed.ncbi.nlm.nih.gov/23262074/ 
• Bioengineering murine mastocytoma cells to produce anticoagulant heparin. L. Gasimli, C. A. Glass, P. Datta, B. Yang, G. Li, T. R. Gemmill, J. Y. Baik, S. T. Sharfstein, J. D. Esko, R. J. Linhardt, Glycobiology. pubmed.ncbi.nlm.nih.gov/24326668/
• Cell-based microscale isolation of glycosaminoglycans for glycomics study. X. Zhao, B. Yang, P. Datta, L. Gasimli, F. Zhang, R. J. Linhardt, Journal of Carbohydrate Chemistry. pubmed.ncbi.nlm.nih.gov/24068855/
• Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin. J. Y. Baik, L. Gasimli, B. Yang, P. Datta, F. Zhang, C. A. Glass, J. D. Esko, R. J. Linhardt, S. T. Sharfstein, Metabolic engineering. pubmed.ncbi.nlm.nih.gov/22326251/

Selected Lists of Abstracts and Presentations

• Optimization of a bioprocess for production of a non-protein biopharmaceutical: Production of a bioengineered heparin. S. T. Sharfstein, J. Y. Baik, L. Talman, E. Oduah, B. Yang, G. Li, F. Zhang, P. Datta, R. J. Linhardt. Book of Abstracts 247th American Chemical Society National Meeting and Exposition. 2014
• Cell engineering heparin and heparan sulfate pathway in mammalian cell line. P. Datta, B. Yang, L. Gasimli, J. Y.Baik, J. D. Esko, R. J. Linhardt, S. T. Sharfstein. National Meeting of the American Chemical Society, New Orleans, LA. April 2013
• Disaccharide compositional analysis of glycosaminoglycans from biological tissues and cells. B. Yang, A. Weyers, J. Y. Baik, S. T. Sharfstein, P. Datta, H. E. Stansfield, R. J. Linhardt. Book of Abstracts 244th American Chemical Society Meeting and Exposition. 2012
• Synthesis of bioengineered heparin in mammalian cell lines. P. Datta, L. Gasimli, X. Zhao, B. Yang, J. Y. Baik, C. A. Glass, J. D. Esko, F. Zhang, S. T. Sharfstein, R. J. Linhardt. Book of Abstracts 244th ACS National Meeting and Exposition. 2012
• Cell based microscale isolation of glycosaminoglycans for glycomics study. X. Zhao, B. Yang, F. Zhang, P. Datta, L. Gasimli, R. J. Linhardt. Book of Abstracts 243rd American Chemical Society National Meeting and Exposition. 2012