- Postdoctoral Fellowship, HIV Pathogenesis, University of Rochester Medical Center, Rochester, NY
- Postdoctoral Fellowship, Genomic Imprinting, Roswell Park Comprehensive Cancer Center, Buffalo, NY
- Ph.D. in Biotechnology, Savitribai Phule Pune University, India.
COURSES TAUGHT AT ACPHS
- Journal Club (BIO660G)
Dr. Singh has an extensive scientific background in studying molecular mechanisms associated with HIV infection and pathogenesis, genomic imprinting, molecular biology and mouse models of disease. Dr. Singh’s research interests include understanding the underlying molecular mechanisms involved in- i) HIV associated neurological disorder, ii) HIV latency, and iii) Viral infection induced developmental defects. Two of his lab’s major projects are listed below.
Project 1: HIV associated comorbidities (neurological disorders): Introduction of combined antiretroviral therapy (cART) has successfully impeded the development of Acquired Immunodeficiency Syndrome (AIDS) and people infected with HIV are living near-normal life span. Unfortunately, various reasons, including cART toxicity and low, albeit persistent viral reservoirs, put these individuals at a higher risk of developing age associated secondary disorders such as cardiovascular disease, neurological disorder, kidney disease and cancer. Dr. Singh’s recent studies have established that HIV can disrupt Blood-Brain-Barrier (BBB) integrity by specifically downregulating Sonic hedgehog (Shh) signaling in brain. BBB disruption is the key event leading to onset of neuroinflammation fueled by the infiltration of HIV infected/activated immune cells in the brain tissue. Dr. Singh’s ongoing research is focused at acquiring a deeper understanding of the roles played by Shh signaling in regulating brain homeostasis specifically in response to viral infection. This line of work presents potential opportunities to design adjunct therapeutic strategies to alleviate viral infection (e.g. HIV, HTLV, HSV, JC Virus, and Zika) induced neuropathogenesis.
Project 2: Research towards HIV cure: eliminating viral reservoirs: The biggest obstacle in achieving cure for HIV is the latent viral reservoirs which are established very early following the infection. There is an inherent heterogeneity among HIV-1 latent reservoirs presumably due to multiple epigenetic mechanisms that drive viral latency, cellular longevity and modulation of host immune responses. It is for this reason that most of the Latency Reversing Agents (LRAs), which worked wonderfully in vitro, were found to be inefficient at affecting viral reservoirs in vivo. Hence, it is important to identify common downstream targets, which are capable of modulating multiple cellular processes in various cell types - long non-coding RNAs (lncRNAs) are among such effectors. Dr. Singh has initiated a novel line of investigation to study the establishment and maintenance of latency orchestrated by multiple partners, including a select class of noncoding RNAs and intrinsic factors of innate immune response that might be working synergistically to keep the integrated provirus in a repressed state. This investigation is supported by exciting preliminary research that was funded by Center for AIDS Research (CFAR, University of Rochester) in the form of a pilot award to Dr. Singh. Successful completion of this study will identify novel targets and pave the way towards HIV cure by ‘Shock and Kill” approach to eliminate latent reservoir.
- Editorial Board member for Scientific reports in the field of infectious diseases.
- Ad-Hoc reviewer for Scientific reports, PLOS One, Journal of Neuroinflammation and BioMed Research International.
- Member of Society on Neuro-Immune Pharmacology (SNIP) since 2013- present.
- Member of Early Career Investigator Award (ECITA) committee for SNIP during 2015-2018.
- Ayse Kizilyer, Meera V Singh, Vir B Singh, James Palis, Sanjay B Maggirwar (2018). Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis. Sci Rep. 2019 Feb 26;9(1):2781. doi: 10.1038/s41598-019-39385-x.
- Singh VB*, Singh MV, Piekna-Przybylska D, Gorantla S, Poluektova LY, Maggirwar SB. Sonic Hedgehog mimetic prevents leukocyte infiltration into the CNS during acute HIV infection. Sci Rep. 2017 Aug 29;7 (1):9578. Doi: 10.1038/s41598-017-10241-0. *Corresponding Author
- Vir B. Singh, Sirinapa Sribenja, Kayla E. Wilson, Kristopher M. Attwood, Joanna C. Hillman, Shilpa Pathak and Michael J Higgins. Blocked transcription through KvDMR1results in absence of methylation and gene silencing resembling Beckwith-Wiedmann syndrome. Development 2017 May 15; 144 (10) :1820-1830. doi: 10.1242/dev.145136. Epub 2017 Apr 20.
- Singh MV, Weber EA, Singh VB, Stirpe NE, Maggirwar SB. Preventive and therapeutic challenges in combating Zika virus infection: are we getting any closer? J Neurovirol. 2017 Jan 23.
- Jackson JW, Singh MV, Singh VB, Jones LD, Davidson GA, Ture S, Morrell CN, Schifitto G, Maggirwar SB. Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis. PLoS One. 2016, June 6; 11(6).