Department


Pharmaceutical Sciences

Associate Professor
Focus: Epigenetics

Contact Information
(802) 735-2634
stanley.stevens@acphs.edu


Speaker Request
Stanley Stevens, Ph.D.

EDUCATION

  • Ph.D. in Chemistry, University of Florida
  • B.S. in Chemistry, University of Central Florida

PREVIOUS POSITIONS OR APPOINTMENTS

  • Associate Professor and Research Director, Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL
  • Research Assistant Professor and Associate Director of the Advanced Mass Spectrometry Laboratory, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX
  • Scientific Director, Proteomics Core, University of Florida, Gainesville, FL

RESEARCH INTERESTS

  • Mass spectrometry (MS)-based proteomics has become a powerful, unbiased approach to understand the complex molecular mechanisms underlying fundamental biological processes as well as mechanisms associated with the development and progression of human disease. This approach is an integral component of our research program at ACPHS Vermont, where we are investigating epigenetics changes that occur in various cell and tissue types after chronic alcohol use. Specifically, we utilize MS to identify and quantify changes in alcohol-induced histone modifications as well as differential protein expression on a global scale in cell culture and animal models of acute and chronic alcohol exposure. In conjunction with bioinformatics, MS-based proteomic data is used to not only determine novel proteins and/or pathways affected by alcohol exposure, but also to predict the activity of potential upstream regulators (e.g., transcription factors and epigenetic modifiers) and downstream functional outcomes, which are then validated by targeted approaches.

    Our currently NIH-funded research projects will investigate the role of microglia, the resident immune cell of the brain, in alcohol-induced neurotoxicity with specific focus on utilizing mass spectrometry-based proteomics to accurately classify activation phenotype in acute and chronic alcohol exposure models and determine the role of histone methylation in regulating alcohol-induced microglial activation phenotype (see current research funding below).

CURRENT RESEARCH FUNDING (as of March 2018)

Grant #: R01AA026082
Funding Agency: NIH/National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Term: 09/01/17 – 06/30/22
Title: Role of histone demethylase KDM5B in ethanol-induced microglial activation
This project aims to investigate the role of a histone demethylase in microglial activation and determine the impact of KDM5B-mediated histone methylation and related functional changes that occur during short- and long-term ethanol exposure.
Role: Principal Investigator (PI)

* * * * *

Grant #: R21AA025183
Funding Agency: NIH/National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Term: 05/01/17- 04/30/19
Title: Role of methylation in ethanol-induced microglial activation
A multi-disciplinary team has been assembled in order to define the role of histone methylation in ethanol-mediated microglial activation and also investigate the effect of ethanol-induced damage of surrounding neurons in this process.
Role: Principal Investigator (multi-PI with Dr. Bin Liu at Department of Pharmacodynamics, University of Florida)

SELECTED BOOK CHAPTERS, PUBLICATIONS, AND POSTERS

    Book Chapters

    Kriss CL, Gregory-Lott E, Storey AJ, Tackett AJ, Wahls WP, Stevens Jr. SM. In vivo metabolic tracing demonstrates the site-specific contribution of hepatic ethanol metabolism to histone acetylation. Alcoholism: Clinical and Experimental Research, 42(10):1909-1923 (2018).

    Rockfield J, Guergues J, Rehman N, Smith A, Bauckman KA, Stevens Jr. SM, Nanjundan M. Proteomic Profiling of Iron-Treated Serous Ovarian Cancer Cells Identifies AKT Activation, which Upregulates the CLEAR network. Proteomics, 18(23):e1800244 (2018).

    Witas, R.; Chaput, D.; Khan, H.; Stevens Jr., S. M. (shared); Kang, D.; Isolation and Proteomic Analysis of Microvesicles and Exosomes from HT22 Cells and Primary Neurons”; Methods Mol Biol. 1598:255-267 (2017).

    Zhang, P.; Culver-Cochran, A.; Stevens Jr., S. M. (shared), Liu B.; “De Novo and Uninterrupted SILAC Labeling of Primary Microglia”; Methods Mol Biol. 1598:285-293 (2017).

    Pinho J.P.C.; Bell-Temin, H.; Liu, B.; Stevens Jr., S. M.; “Spike-In SILAC Approach for Proteomic Analysis of Ex Vivo Microglia”; Methods Mol Biol. 1598:295-312 (2017).

    Bell-Temin, H.; Zhang, P.; Liu, B.; Stevens Jr., S. M.; “Biomarkers for drug abuse-induced brain injury: role of microglia in alcohol-induced neurotoxicity”; Biomarkers of Brain Injury & Brain Disorders; Wang, Kevin K. W., Ed, Zhang, Zhiqun, Ed and Kobaissy, Firas, Ed; Science Publishers; Enfield, NH (2014).

    Maldonado-Devincci, A. M.; Stevens Jr., S. M (shared).; Kirstein, C. L.; “Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure”; in Psychiatric Disorder: Methods and Protocols; Kobaissy, Firas, Ed; Humana Press Inc.: Totowa, NJ (2012).

    Liu, B.; Barber, D. S.; Stevens Jr., S. M.; “Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)-based Proteomic Analysis of Ethanol-Induced Protein Expression Profiles in Microglia”; in Psychiatric Disorder: Methods and Protocols; Kobaissy, Firas, Ed; Humana Press Inc.: Totowa, NJ (2012).

    Prokai, L.; Stevens Jr., S. M.; Simonsick Jr., W. J.; “Gel Permeation Chromatography Coupled to Mass Spectrometry for Oligomer Analysis”; in Multiple Detection in Size-Exclusion Chromatography; Striegel, André, Ed; American Chemical Society: Washington, DC (2004).

    Peer Reviewed Journal Articles

    Flowers, A.; Bell-Temin, H.; Jalloh, A.; Stevens, Jr., S. M.; Paula C. Bickford; “Proteomic Analysis of Aged Microglia: Shifts in Transcription, Bioenergetics, and Nutrient Response”; J. Neuroinflammation, 14(1):96 (2017).

    Scott, K. T.; Mangiapia, M.; Brown, T.-R.; Chaput, D.; Haller, E.; Harmer, T.; Hashemy, Z.; Keeley, R.; Leonard, J.; Mancera,  P.; Nicholson, P.; Stevens, Jr., S. M.; Wanjugi, P.; Zabinski, T.; Pan, C.; “Proteomic and mutant analysis of the CO2 concentrating mechanism of hydrothermal vent chemolithoautotroph Thiomicrospira crunogena”; J. Bacteriology, 199(7). pii: e00871-16 (2017).

    Sanchez, A.; De Vivo, A.; Uprety, N.; Kim, J.; Stevens Jr., S. M.; Kee, Y.; “The BMI1-UBR5 axis regulates transcriptional repression at damaged chromatin”; PNAS, 113:11243-11248 (2016).

    Athanason, M. G., Stevens, Jr., S. M., Burkhardt, B. R.; “Hepatic SILAC Proteomic Data from PANDER transgenic model”; Data in Brief, 9:159-62 (2016).

    Athanason, M. G., Ratliff, W. A., Chaput, D., MarElia, C. B., Kuehl, M. N., Stevens, Jr., S. M., Burkhardt, B. R.; “Quantitative proteomic profiling reveals hepatic lipogenesis and liver X receptor activation in the PANDER transgenic model”; Mol. Cell. Endocrinol., 436, 41-49 (2016).

    Fontaine, S. N.; Sabbagh, J. J.; Martin, M.; Chaput, D.; Darling, A.; Trotter, J.; Zheng, D.; Stothert, A.; Nordhues, B.; Lussier, A.; Baker, J.; Shelton, L.; Blair, L.; Stevens, Jr., S. M.; Dickey, C. A.; “DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins”; EMBO., 35, 1537-1549 (2016).

    Bahia, P. K.; Parks, T. A.; Stanford, K. R.; Mitchell, D. A.; Varma, S.; Stevens, Jr., S. M.; Taylor-Clark, T. E.; “The exceptionally high reactivity of Cys 621 is critical for electrophilic activation of TRPA1”; J. Gen. Physiol., 147, 451-465 (2016).

    Narayan, M.; Kirouac, L.; Chaput, D.; Stevens, Jr., S. M.; Padmanabhan, J.; Jinwal, U. K.; “Identification of Novel Cdc37 Interacting Proteins and Pathways in Human Alzheimer's Disease Brain Tissue using Mass Spectrometry”; J. Data Mining Genomics Proteomics, 7(2): 1000193 (2016).

    Zhang, P.*; Cochran-Culver, A.*; Stevens, Jr., S. M. (shared last author); Liu, B.; “Characterization of a SILAC method for proteomic analysis of primary rat microglia”; Proteomics, 16, 1341-1346 (2016).

    Martin, D. R.; Dutta, P.; Mahajan, S.; Varma, S.; Stevens, Jr., S. M.; “Structural and activity characterization of human PHPT1 after oxidative modification”; Nat. Sci. Rep., 6: 23658 (2016).

    Chaput, D.; Kirouac, L. H.; Bell-Temin, H.; Stevens, Jr., S. M. (shared last author); Padmanabhan, J.; “Potential role of PCTAIRE-2, PCTAIRE-3 and P-Histone H4 in amyloid precursor protein-dependent Alzheimer pathology”; Oncotarget, 7, 8481-8497 (2016). 

    Prokai, L.; Stevens, Jr., S. M.; “Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry”; Int. J. Mol. Sci., 17(1). pii: E116 (2016).

    Posters

    Kriss, C.*; Stevens Jr., S. M.; “Metabolic tracing in hepatocytes demonstrates the contribution of acetyl-CoA derived from ethanol metabolism to histone acetylation”; 65th ASMS Conference on Mass Spectrometry and Allied Topics; Indianapolis, 2017.

    Stevens Jr., S. M.; “Role of phosphohistidine signaling in ethanol-induced hepatic steatosis”; 10th International Congress of the NDP Kinase/NM23/AWD Gene Family; Dubrovnik, Croatia, 2016.

    Pinho, J. P. C.*; Guergues, J.; Bell-Temin, H.; Liu, B.; Stevens Jr., S. M.; “Mass spectrometry-based characterization of histone methylation in microglia after ethanol exposure”; 63rd ASMS Conference on Mass Spectrometry and Allied Topics; St. Louis, 2015.

    Stevens Jr., S. M.; “Relationship between Alcohol-induced Oxidative Stress and Histone Modifications”; University of Pittsburgh; Pittsburgh, 2014.

    Stevens Jr., S. M.; “Alcohol-induced Oxidative Stress: Insights from Mass Spectrometry-Based Proteomics”; University of Mississippi Medical Center; Jackson, 2014.

    Stevens Jr., S. M.; “Cellular Response to Alcohol-induced Oxidative Stress: Insights from Mass Spectrometry-Based Proteomics”; University of South Florida; Tampa, 2013.

    Stevens Jr., S. M.; “Cellular Response to Alcohol-induced Oxidative Stress: Insights from Mass Spectrometry-Based Proteomics”; University of Florida; Gainesville, 2013.

    Stevens Jr., S. M.; “Identification of Ethanol-Induced Pathway Alterations in Microglial Cells using Mass Spectrometry-Based Proteomics”; Virginia Commonwealth University; Richmond, 2012.

    Stevens Jr., S. M.; “New Insights into the Selectivity and Functional Impact of Oxidative Stress-Induced Protein Modifications”; Banyan Biomarkers; Alachua, 2011.

    Stevens Jr., S. M.; “Identification of Novel Alcohol-induced Pathway Alterations by SILAC and iTRAQ-based Quantitative Proteomics”; NuSep Users Meeting; 59th ASMS Conference on Mass Spectrometry and Allied Topics; Denver, 2011.

    CONFERENCES AND PRESENTATIONS

    Guergues J, Rath M, Pinho JP, Nguyen TG, Peris J, McLaughlin JP, MacFdyen K, Zhang P, Liu B, Stevens Jr. SM. Super-SILAC Analysis Reveals Sex Differences in Alcohol-Induced Microglial Activation: Potential Link to Anxiety Development After Chronic Alcohol Exposure in Mice. 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego CA, 2018.

    Kriss C, Gregory-Lott E, Storey AJ, Stevens Jr. SM. In Vivo Metabolic Tracing Reveals Site-Specific Contribution of Hepatic Ethanol Metabolism to Histone Acetylation. 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego CA, 2018.

    Stevens Jr. SM. Applications of Stable Isotope Labeling in MS, ASMS Conference, San Diego CA, 2018 (session chair).

    EXTRAMURAL GRANTS 

    Role: Principal Investigator
    Project Title: The role of histone demethylase KDM5B in ethanol-induced microglial activation
    Grantor: National Institute on Alcohol Abuse and Alcoholism
    Grant Number: 5R01AA026082-03
    Amount: $379,402
    Term: 7/01/2019 - 6/30/2019

    Role: Principal Investigator
    Project Title: Role of methylation in ethanol-induced microglial activation
    Grantor: National Institute on Alcohol Abuse and Alcoholism
    Grant Number: 7R21AA025183-02
    Amount: $278,000
    Term: 5/01/2018 - 4/30/2019

    HONORS, AWARDS, AND APPOINTMENTS

    Ad Hoc reviewer for NIH, Neurotoxicity and Alcohol Study Section (Feb. 22-23, 2018)