Arcadius V. Krivoshein, Ph.D.
Ph.D. in Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
My research interests lie in the areas of mechanistic biochemistry and molecular pharmacology. Two main questions we are asking in my laboratory are:
- What is the relationship between structure and activity among antiepileptic drugs? We recently discovered that compounds containing an α-substituted amide group produce their antiepileptic effect at least in part by inhibiting the neuronal nicotinic acetylcholine receptors. An interdisciplinary approach combining rapid chemical kinetics, spectroscopy, chiral HPLC and, most recently, X-ray diffraction is being used to elucidate the mechanism of action of these drugs.
- Are there endogenous small molecules in blood that modulate aggregation of amyloid β peptides? We are using liquid chromatography, tandem mass spectrometry, and Aβ(1-40) peptide aggregation assays to isolate and identify such molecules from mammalian and human blood serum.
These projects involve collaboration with scientists at New Mexico Highlands University, Hawaii Pacific University, and National Institute of Neurological Disorders and Stroke – NIH.
Click here to learn more about Dr. Krivoshein's Research Group.
Eder, M., Krivoshein, A. V., Backer, M. V., Backer, J. M., Haberkorn, U., and Eisenhut, M. (2010) scVEGF-PEG-HBED-CC and scVEGF-PEG-NOTA conjugates: comparison of easy-to-label recombinant proteins for [68Ga]PET imaging of VEGF receptors in angiogenic vasculature. Nucl. Med. Biol. 37, 405-412.
Alexander, J. K., Sagher, D., Krivoshein, A. V., Criado, M., Jefford, G., and Green, W. N. (2010) Ric-3 promotes α7 nicotinic receptor assembly and trafficking through the ER sub-compartment of dendrites. J. Neurosci. 30, 10112-10126.
Backer, J. M., Krivoshein, A. V., Hamby, C. V., Pizzonia, J., Gilbert, K., Ray, Y. S., Brand, H., Paton, A. W., Paton, J. C., and Backer, M. V. (2009) Chaperone-targeting cytotoxin and endoplasmic reticulum stress-inducing drug synergize to kill cancer cells. Neoplasia 11, 1-9.
Krivoshein, A. V., and Hess, G. P. (2006) On the mechanism of alleviation by phenobarbital of the malfunction of an epilepsy-linked GABAA receptor. Biochemistry 45, 11632-11641.
Krivoshein, A. V., and Hess, G. P. (2004) A mechanism-based approach to the successful prevention of cocaine inhibition of the neuronal (α3β4) nicotinic acetylcholine receptor. Biochemistry 43, 481-489.